VAMP2 Monoklonaler Antikörper

VAMP2 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G7E8

Kat-Nr. : 67822-1-PBS

Synonyme

FLJ11460, SYB2, Synaptobrevin 2, VAMP 2, VAMP2

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  WB analysis using 67822-1-Ig (same clone as 67822-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67822-1-Ig (VAMP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67822-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67822-1-Ig (same clone as 67822-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67822-1-Ig (VAMP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67822-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67822-1-Ig (same clone as 67822-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67822-1-Ig (VAMP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67822-1-PBS in a different storage buffer formulation.

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  IF Staining of U-87 MG using 67822-1-Ig (same clone as 67822-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using VAMP2 antibody (67822-1-Ig, Clone: 1G7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67822-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 67822-1-Ig (same clone as 67822-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using VAMP2 antibody (67822-1-Ig, Clone: 1G7E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67822-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67822-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA VAMP2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VAMP2 fusion protein Ag17908
• Vollständiger Name: vesicle-associated membrane protein 2 (synaptobrevin 2)
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC002737
• Gene symbol: VAMP2
• Gene ID (NCBI): 6844
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VAMP2 (vesicle-associated membrane protein 2), also named as synaptobrevin 2, is a member of the SNARE (soluble NSF-attachment protein receptor) family proteins. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP2, with a molecular mass of 15-19 kDa, consists of a short N-terminal sequence, a SNARE motif, and a C-terminal transmembrane region. It is required for fast calcium-triggered synaptic vesicle fusion. VAMP2 forms a stable complex with STX1 (syntaxin 1) and SNAP25 (synaptosomal-associated protein 25) during synaptic vesicle fusion (PMID: 16793874). It also forms a distinct complex with synaptophysin. VAMP2 is expressed in nervous system and some non-neuronal tissues, such as skeletal muscle (PMID: 18570252).