VAMP3/Cellubrevin Polyklonaler Antikörper

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• KD/KO Validated

VAMP3/Cellubrevin Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10702-1-PBS

Synonyme

VAMP3, CEB, Cellubrevin, SYB3, Synaptobrevin-3

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  WB analysis of HEK-293 using 10702-1-AP (same clone as 10702-1-PBS)

  WB result of VAMP3/Cellubrevin antibody (10702-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VAMP3/Cellubrevin transfected HEK-293 cells. This data was developed using the same antibody clone with 10702-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10702-1-AP (same clone as 10702-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10702-1-AP (VAMP3/Cellubrevin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10702-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 10702-1-AP (same clone as 10702-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10702-1-AP (VAMP3/Cellubrevin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10702-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 10702-1-AP (same clone as 10702-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10702-1-AP (VAMP3/Cellubrevin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10702-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 10702-1-AP (same clone as 10702-1-PBS)

  IP result of anti-VAMP3/Cellubrevin (IP:10702-1-AP, 4ug; Detection:10702-1-AP 1:1000) with HEK-293 cells lysate 3000ug. This data was developed using the same antibody clone with 10702-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 10702-1-AP (same clone as 10702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10702-1-AP (VAMP3/Cellubrevin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10702-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 10702-1-AP (same clone as 10702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10702-1-AP (VAMP3/Cellubrevin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10702-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 10702-1-AP (same clone as 10702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10702-1-AP (VAMP3/Cellubrevin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10702-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10702-1-PBS bindet in WB, IHC, IP, Indirect ELISA VAMP3/Cellubrevin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VAMP3/Cellubrevin fusion protein Ag1156
• Vollständiger Name: vesicle-associated membrane protein 3 (cellubrevin)
• Berechnetes Molekulargewicht: 11 kDa
• Beobachtetes Molekulargewicht: 11-17 kDa
• GenBank-Zugangsnummer: BC005941
• Gene symbol: VAMP3
• Gene ID (NCBI): 9341
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VAMP3, also named as cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.