VAMP3/Cellubrevin Monoklonaler Antikörper

VAMP3/Cellubrevin Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1D10D10

Kat-Nr. : 66488-1-Ig

Synonyme

VAMP3, Synaptobrevin-3, SYB3, Cellubrevin, CEB

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  WB analysis of pig brain using 66488-1-Ig

  Pig brain, rat brain and mouse brain tissues were subjected to SDS PAGE followed by western blot with 66488-1-Ig (VAMP3/Cellubrevin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66488-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66488-1-Ig (VAMP3/Cellubrevin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Neuro-2a using 66488-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using VAMP3/Cellubrevin antibody (66488-1-Ig, Clone: 1D10D10 ) at dilution of 1:1800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, HEK-293-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: Neuro-2a-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:900-1:3600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66488-1-Ig bindet in WB, IF/ICC, ELISA VAMP3/Cellubrevin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VAMP3/Cellubrevin fusion protein Ag1156
• Vollständiger Name: vesicle-associated membrane protein 3 (cellubrevin)
• Berechnetes Molekulargewicht: 11 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC005941
• Gene symbol: VAMP3
• Gene ID (NCBI): 9341
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VAMP3, also named cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.

Publikationen

Species	Application	Title
human	WB	J Exp Clin Cancer Res; Long non-coding RNA NORAD promotes the prostate cancer cell extracellular vesicle release via microRNA-541-3p-regulated PKM2 to induce bone metastasis of prostate cancer.; Authors - Chuan-Yi Hu