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VAMP3/Cellubrevin Monoklonaler Antikörper

VAMP3/Cellubrevin Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D10D10

Kat-Nr. : 66488-1-PBS

Synonyme

VAMP3, Synaptobrevin-3, SYB3, Cellubrevin, CEB

Filter:
  • Western Blot (WB) analysis of pig brain tissue using VAMP3/Cellubrevin Monoclonal antibody (66488-1-Ig)

    WB analysis of pig brain using 66488-1-Ig (same clone as 66488-1-PBS)

    Pig brain, rat brain and mouse brain tissues were subjected to SDS PAGE followed by western blot with 66488-1-Ig (VAMP3/Cellubrevin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66488-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using VAMP3/Cellubrevin Monoclonal antibody (66488-1-Ig)

    WB analysis of HEK-293 using 66488-1-Ig (same clone as 66488-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66488-1-Ig (VAMP3/Cellubrevin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66488-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using VAMP3/Cellubrevin Monoclonal antibody (66488-1-Ig)

    IF Staining of Neuro-2a using 66488-1-Ig (same clone as 66488-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using VAMP3/Cellubrevin antibody (66488-1-Ig, Clone: 1D10D10 ) at dilution of 1:1800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66488-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66488-1-PBS bindet in WB, IF/ICC, Indirect ELISA VAMP3/Cellubrevin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen VAMP3/Cellubrevin fusion protein Ag1156
Vollständiger Name vesicle-associated membrane protein 3 (cellubrevin)
Berechnetes Molekulargewicht 11 kDa
Beobachtetes Molekulargewicht 17 kDa
GenBank-ZugangsnummerBC005941
Gene symbol VAMP3
Gene ID (NCBI) 9341
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VAMP3, also named cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.