VAMP4 Monoklonaler Antikörper

VAMP4 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3A6F2

Kat-Nr. : 67219-1-PBS

Synonyme

3A6F2, VAMP-4, Vesicle-associated membrane protein 4

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  WB analysis using 67219-1-Ig (same clone as 67219-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67219-1-Ig (VAMP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67219-1-Ig (same clone as 67219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67219-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67219-1-Ig (same clone as 67219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67219-1-Ig (same clone as 67219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67219-1-Ig (same clone as 67219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67219-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 67219-1-Ig (same clone as 67219-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 67219-1-Ig (VAMP4 antibody), at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67219-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67219-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA VAMP4 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VAMP4 fusion protein Ag27854
• Vollständiger Name: vesicle-associated membrane protein 4
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC007019
• Gene symbol: VAMP4
• Gene ID (NCBI): 8674
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VAMP4 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP4 may play a role in trans-Golgi network to endosome transport.