VAMP7/TI-VAMP Polyklonaler Antikörper

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• KD/KO Validated

VAMP7/TI-VAMP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22268-1-PBS

Synonyme

VAMP7, TI VAMP, Tetanus-insensitive VAMP, Tetanus insensitive VAMP, Synaptobrevin-like protein 1

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  WB analysis using 22268-1-AP (same clone as 22268-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 22268-1-AP (same clone as 22268-1-PBS)

  IP result of anti-VAMP7/TI-VAMP (IP:22268-1-AP, 4ug; Detection:22268-1-AP 1:1000) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 22268-1-AP (same clone as 22268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 22268-1-AP (same clone as 22268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 22268-1-AP (same clone as 22268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 22268-1-AP (same clone as 22268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 22268-1-AP (same clone as 22268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 22268-1-AP (same clone as 22268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 22268-1-AP (same clone as 22268-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAMP7/TI-VAMP antibody (22268-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 22268-1-AP (same clone as 22268-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22268-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA VAMP7/TI-VAMP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: vesicle-associated membrane protein 7
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: NM_005638
• Gene symbol: VAMP7
• Gene ID (NCBI): 6845
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VAMP7, also named SYBL1 and TI-VAMP, belongs to the synaptobrevin family. It is involved in the targeting and/or fusion of transport vesicles to their target membrane during the transport of proteins from the early endosome to the lysosome. VAMP7 is required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. It is necessary for calcium-regulated lysosomal exocytosis. VAMP7 is involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. It is required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. It is also required for focal exocytosis of late endocytic vesicles during phagosome formation. The antibody is specific to VAMP7.