VANGL2 Polyklonaler Antikörper

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• KD/KO Validated

VANGL2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21492-1-PBS

Synonyme

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  WB analysis using 21492-1-AP (same clone as 21492-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21492-1-AP (VANGL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21492-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 21492-1-AP (same clone as 21492-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21492-1-AP (VANGL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21492-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 21492-1-AP (same clone as 21492-1-PBS)

  IP result of anti-VANGL2 (IP:21492-1-AP, 4ug; Detection:21492-1-AP 1:500) with HepG2 cells lysate 1880 ug. This data was developed using the same antibody clone with 21492-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 21492-1-AP (same clone as 21492-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21492-1-AP (VANGL2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21492-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 21492-1-AP (same clone as 21492-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21492-1-AP (VANGL2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21492-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 21492-1-AP (same clone as 21492-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 21492-1-AP (VANGL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21492-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21492-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA VANGL2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VANGL2 fusion protein Ag15833
• Vollständiger Name: vang-like 2 (van gogh, Drosophila)
• Berechnetes Molekulargewicht: 521 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC103920
• Gene symbol: VANGL2
• Gene ID (NCBI): 57216
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vangl2 is a key component of the planar cell polarity (PCP) pathway. Vangl2 is tightly associated with the postsynaptic density (PSD) fraction and forms a protein complex with PSD-95 and NMDA receptors. Vangl2 directly binds to the third PDZ domain of PSD-95 via its C-terminal TSV motif. Vangl2 directly binds to N-cadherin.