VANGL2 Monoklonaler Antikörper

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• KD/KO Validated

VANGL2 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1B2C1

Kat-Nr. : 67273-1-Ig

Synonyme

KIAA1215, Loop tail protein 1 homolog, LPP1, LTAP, STB1, STBM, STBM1, Strabismus 1, Van Gogh like protein 2, Vang like protein 2, VANGL2

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  WB analysis of MCF-7 using 67273-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67273-1-Ig (VANGL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67273-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67273-1-Ig (VANGL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67273-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67273-1-Ig (VANGL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

67273-1-Ig bindet in WB, ELISA VANGL2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: hamster
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VANGL2 fusion protein Ag16980
• Vollständiger Name: vang-like 2 (van gogh, Drosophila)
• Berechnetes Molekulargewicht: 521 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC103920
• Gene symbol: VANGL2
• Gene ID (NCBI): 57216
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vangl2 is a key component of the planar cell polarity (PCP) pathway. Vangl2 is tightly associated with the postsynaptic density (PSD) fraction and forms a protein complex with PSD-95 and NMDA receptors. Vangl2 directly binds to the third PDZ domain of PSD-95 via its C-terminal TSV motif. Vangl2 directly binds to N-cadherin.

Publikationen

Species	Application	Title
hamster	WB	Biochim Biophys Acta Gene Regul Mech; MicroRNA-27a-3p targeting Vangl1 and Vangl2 inhibits cell proliferation in mouse granulosa cells; Authors - Hu Tao ; KD Validated