VAPA Polyklonaler Antikörper

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VAPA Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15275-1-PBS

Synonyme

33 kDa VAMP associated protein, 33 kDa VAMP-associated protein, hVAP 33, VAMP A, VAMP associated protein A

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  WB analysis using 15275-1-AP (same clone as 15275-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

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  WB analysis using 15275-1-AP (same clone as 15275-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 15275-1-AP (same clone as 15275-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 15275-1-AP (same clone as 15275-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 15275-1-AP (same clone as 15275-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15275-1-AP (same clone as 15275-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 15275-1-AP (same clone as 15275-1-PBS)

  IP result of anti-VAPA (IP:15275-1-AP, 3ug; Detection:15275-1-AP 1:1000) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15275-1-AP (same clone as 15275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15275-1-AP (same clone as 15275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 15275-1-AP (same clone as 15275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15275-1-AP (same clone as 15275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15275-1-AP (same clone as 15275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 15275-1-AP (same clone as 15275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 15275-1-AP (same clone as 15275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 15275-1-AP (same clone as 15275-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAPA antibody (15275-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 15275-1-AP (same clone as 15275-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAPA antibody (15275-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 15275-1-AP (same clone as 15275-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 15275-1-AP (VAPA antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15275-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA VAPA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VAPA fusion protein Ag7393
• Vollständiger Name: VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC002992
• Gene symbol: VAPA
• Gene ID (NCBI): 9218
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.