VAPA Monoklonaler Antikörper

VAPA Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F10F9

Kat-Nr. : 67845-1-PBS

Synonyme

33 kDa VAMP associated protein, hVAP 33, VAMP A, VAMP associated protein A, VAP 33, VAP A, VAP33, VAPA

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  WB analysis using 67845-1-Ig (same clone as 67845-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67845-1-Ig (VAPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67845-1-Ig (same clone as 67845-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67845-1-Ig (same clone as 67845-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67845-1-Ig (same clone as 67845-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67845-1-Ig (same clone as 67845-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67845-1-Ig (VAPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67845-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67845-1-PBS bindet in WB, IHC, Indirect ELISA VAPA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VAPA fusion protein Ag7819
• Vollständiger Name: VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC002992
• Gene symbol: VAPA
• Gene ID (NCBI): 9218
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a 33-kDa, ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.