VAPB Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

VAPB Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(26)

CloneNo.

4F6A6

Kat-Nr. : 66191-1-Ig

Synonyme

4F6A6, ALS8, UNQ484/PRO983, VAMP associated protein B/C, VAMP B

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  WB analysis of HepG2 using 66191-1-Ig

  WB result of VAPB antibody (66191-1-Ig, 1:5000) with si-control and si-VAPB transfected HepG2 cells.

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  WB analysis using 66191-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66191-1-Ig (VAPB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66191-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66191-1-Ig (VAPB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66191-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66191-1-Ig (VAPB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 66191-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66191-1-Ig (VAPB Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 66191-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66191-1-Ig (VAPB antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66191-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAPB antibody (66191-1-Ig, Clone: 4F6A6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 66191-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66191-1-Ig (VAPB antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 14 publications below
• IF: See 10 publications below
• CoIP: See 3 publications below

Produktinformation

66191-1-Ig bindet in WB, IHC, IF/ICC, CoIP, ELISA VAPB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Hefe, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VAPB fusion protein Ag6313
• Vollständiger Name: VAMP (vesicle-associated membrane protein)-associated protein B and C
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 30-32 kDa
• GenBank-Zugangsnummer: BC001712
• Gene symbol: VAPB
• Gene ID (NCBI): 9217
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.

Publikationen

Species	Application	Title
human	WB	EMBO J; CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.; Authors - Thaddaeus Mutugi Nthiga
monkey	WB,IF,CoIP	Curr Biol; The ER Contact Proteins VAPA/B Interact with Multiple Autophagy Proteins to Modulate Autophagosome Biogenesis.; Authors - Yan G Zhao ; KD Validated
human	WB	J Cell Biol; Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.; Authors - Nan Liu
human	WB,CoIP	EMBO Rep; Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling; Authors - Misha Kalarikkal ; KD Validated
human	WB	J Biol Chem; Proteomic mapping by rapamycin-dependent targeting of APEX2 identifies binding partners of VAPB at the inner nuclear membrane.; Authors - Christina James
human	CoIP	J Biochem; MITOL regulates phosphatidic acid-binding activity of RMDN3/PTPIP51.; Authors - Naoki Ito