VAPB Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

VAPB Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4F6A6

Kat-Nr. : 66191-1-PBS

Synonyme

4F6A6, ALS8, UNQ484/PRO983, VAMP associated protein B/C, VAMP B

---

• 

  WB analysis of HepG2 using 66191-1-Ig (same clone as 66191-1-PBS)

  WB result of VAPB antibody (66191-1-Ig, 1:5000) with si-control and si-VAPB transfected HepG2 cells. This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66191-1-Ig (same clone as 66191-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66191-1-Ig (VAPB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66191-1-Ig (same clone as 66191-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66191-1-Ig (VAPB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66191-1-Ig (same clone as 66191-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66191-1-Ig (VAPB Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66191-1-Ig (same clone as 66191-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66191-1-Ig (VAPB Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66191-1-Ig (same clone as 66191-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66191-1-Ig (VAPB antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66191-1-Ig (same clone as 66191-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAPB antibody (66191-1-Ig, Clone: 4F6A6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66191-1-Ig (same clone as 66191-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66191-1-Ig (VAPB antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66191-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA VAPB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VAPB fusion protein Ag6313
• Vollständiger Name: VAMP (vesicle-associated membrane protein)-associated protein B and C
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 30-32 kDa
• GenBank-Zugangsnummer: BC001712
• Gene symbol: VAPB
• Gene ID (NCBI): 9217
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.