VARS2 Polyklonaler Antikörper

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• KD/KO Validated

VARS2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15776-1-PBS

Synonyme

Valyl tRNA synthetase like, ValRS, Valine--tRNA ligase, mitochondrial, Valine tRNA ligase, KIAA1885

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  WB analysis using 15776-1-AP (same clone as 15776-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

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  WB analysis using 15776-1-AP (same clone as 15776-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 15776-1-AP (same clone as 15776-1-PBS)

  IP result of anti-VARS2 (IP:15776-1-AP, 4ug; Detection:15776-1-AP 1:500) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15776-1-AP (same clone as 15776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15776-1-AP (same clone as 15776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 15776-1-AP (same clone as 15776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 15776-1-AP (same clone as 15776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15776-1-AP (same clone as 15776-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VARS2 antibody (15776-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15776-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA VARS2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VARS2 fusion protein Ag8460
• Vollständiger Name: valyl-tRNA synthetase 2, mitochondrial (putative)
• Berechnetes Molekulargewicht: 119 kDa, 57 kDa
• Beobachtetes Molekulargewicht: 118 kDa
• GenBank-Zugangsnummer: BC008844
• Gene symbol: VARS2
• Gene ID (NCBI): 57176
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VARS2(Valine--tRNA ligase, mitochondrial) is also named as KIAA1885, VARS2L, VARSL and belongs to the class-I aminoacyl-tRNA synthetase family. The deduced 993-amino acid protein has an N-terminal mitochondrial targeting signal with a predicted cleavage site after residue 29. VARS2 has characteristics of a class I mitochondrial aminoacyl-tRNA synthetase, including a classical Rossmann fold.