VASP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VASP Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 13472-1-AP

Synonyme

VASP

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Galerie der Validierungsdaten

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  WB analysis using 13472-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13472-1-AP (VASP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 13472-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13472-1-AP (VASP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 13472-1-AP

  IP result of anti-VASP(IP:13472-1-AP, 4ug; Detection:13472-1-AP 1:10000) with HEK-293 cells lysate 920 ug.

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  IHC staining of human testis using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin cancer using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin cancer using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 13472-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using VASP antibody (13472-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 13472-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug VASP antibody (13472-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, C6-Zellen, HEK-293-Zellen, HeLa-Zellen, Mausmilzgewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Hautkrebsgewebe, humanes Herzgewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

13472-1-AP bindet in WB, IP, IHC, IF, FC, ELISA VASP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VASP fusion protein Ag4266
• Vollständiger Name: vasodilator-stimulated phosphoprotein
• Berechnetes Molekulargewicht: 380 aa, 40 kDa
• Beobachtetes Molekulargewicht: 46 kDa, 50 kDa
• GenBank-Zugangsnummer: BC038224
• Gene symbol: VASP
• Gene ID (NCBI): 7408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VASP belongs to the Ena/VASP family. Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. VASP plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. Human platelet activation is inhibited by agents such as prostaglandins and NO donors, which elevate cAMP or cGMP levels. VASP is phosphorylated in human platelets in response to both cAMP- and cGMP-elevating agents, and its phosphorylation correlates with platelet inhibition. VASP is located about 92 kb distal to ERCC1 (126380) and about 300 kb proximal to the myotonic dystrophy protein kinase gene. The antibody is specific to VASP.

Publikationen

Species	Application	Title
human	IF	Nat Commun; Cortical dynamics during cell motility are regulated by CRL3(KLHL21) E3 ubiquitin ligase.; Authors - Thibault Courtheoux
human	WB	PLoS Biol; Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.; Authors - Yajing Lv
human	WB, IF, IHC	Oncogene; The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.; Authors - Kai Li ; KD Validated
human	WB	Front Oncol; High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells.; Authors - Jun Wang
human	WB	Oncol Rep; TNF‑α inhibits xenograft tumor formation by A549 lung cancer cells in nude mice via the HIF‑1α/VASP signaling pathway.; Authors - Wen Liu ; KD Validated
human	WB	Pathol Res Pract; Silencing lncRNA SNHG6 suppresses proliferation and invasion of breast cancer cells through miR-26a/VASP axis.; Authors - Kai Li