VASP Monoklonaler Antikörper

VASP Monoklonal Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B9A11

Kat-Nr. : 68431-1-PBS

Synonyme

1B9A11, Vasodilator-stimulated phosphoprotein

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  WB analysis using 68431-1-Ig (same clone as 68431-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68431-1-Ig (VASP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68431-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array sample test of MP51452-1

  Cytometric bead array sample test of MP51452-1, VASP Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68431-1-PBS. Detection antibody: 68431-2-PBS.

• 

  Cytometric bead array standard curve of MP51452-1

  Cytometric bead array standard curve of MP51452-1, VASP Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68431-1-PBS. Detection antibody: 68431-2-PBS. Standard:Ag17679. Range: 1.563-200 ng/mL.

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Geprüfte Anwendungen

Produktinformation

68431-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA VASP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VASP fusion protein Ag17679
• Vollständiger Name: vasodilator-stimulated phosphoprotein
• Berechnetes Molekulargewicht: 380 aa, 40 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC038224
• Gene symbol: VASP
• Gene ID (NCBI): 7408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VASP belongs to the Ena/VASP family. Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. VASP plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. Human platelet activation is inhibited by agents such as prostaglandins and NO donors, which elevate cAMP or cGMP levels. VASP is phosphorylated in human platelets in response to both cAMP- and cGMP-elevating agents, and its phosphorylation correlates with platelet inhibition. VASP is located about 92 kb distal to ERCC1 (126380) and about 300 kb proximal to the myotonic dystrophy protein kinase gene. The antibody is specific to VASP.