VAV1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VAV1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 16364-1-AP

Synonyme

Proto oncogene vav, Proto-oncogene vav, VAV

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  WB analysis using 16364-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16364-1-AP (VAV1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 16364-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16364-1-AP (VAV1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 16364-1-AP

  IP result of anti-VAV1 (IP:16364-1-AP, 4ug; Detection:16364-1-AP 1:4000) with K-562 cells lysate 1800 ug.

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  IHC staining of human breast cancer using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lymphoma using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Ramos-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hirngewebe, humanes Herzgewebe, humanes Nierengewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Lymphomgewebe, humanes Milzgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

16364-1-AP bindet in WB, IHC, IF, IP, ELISA VAV1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Gecko
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VAV1 fusion protein Ag9049
• Vollständiger Name: vav 1 guanine nucleotide exchange factor
• Berechnetes Molekulargewicht: 845 aa, 98 kDa
• Beobachtetes Molekulargewicht: 98 kDa
• GenBank-Zugangsnummer: BC013361
• Gene symbol: VAV1
• Gene ID (NCBI): 7409
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vav proteins mainly act as enzymes that catalyze the activation step of Rho subfamily GTPases during cell signaling. There are three family members:VAV1, VAV2 and VAV3. Vav1 is specifically expressed in the hematopoietic system, whereas Vav2 and Vav3 are more ubiquitously expressed (PMID:14607270). Vav1 is physiologically active as a GDP/GTP nucleotide exchange factor (GEF) in the hematopoietic system (PMID:31654719).

Publikationen

Species	Application	Title
gecko	WB,IF	J Biol Chem; Adult astrocytes from reptiles are resistant to proinflammatory activation via sustaining Vav1 expression.; Authors - Nan Du ; KD Validated
human	WB,IHC	Biochem Biophys Res Commun; Vav1 expression is increased in esophageal squamous cell carcinoma and indicates poor prognosis.; Authors - Xiaolei Zhu
human	WB	Oncotarget; Multiple regulatory aspects of histone methyltransferase EZH2 in Pb-induced neurotoxicity.; Authors - Wei-Zhen Xue
rat	WB,IF	Clin Sci (Lond); Novel role of Vav1-Rac1 pathway in actin cytoskeleton regulation in Interleukin-13-induced minimal change-like nephropathy.; Authors - Chang-Yien Chan ; KD Validated
human	WB	Cancer Cell Int; STING pathway contributes to the prognosis of hepatocellular carcinoma and identification of prognostic gene signatures correlated to tumor microenvironment; Authors - Zhangya Pu
rat	IF	Neural Regen Res; Vav1 promotes inflammation and neuronal apoptosis in cerebral ischemia/reperfusion injury by upregulating microglial and NLRP3 inflammasome activation; Authors - Jing Qiu