VAV1 Polyklonaler Antikörper

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VAV1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16364-1-PBS

Synonyme

Proto oncogene vav, Proto-oncogene vav, VAV

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  WB analysis using 16364-1-AP (same clone as 16364-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16364-1-AP (VAV1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 16364-1-AP (same clone as 16364-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16364-1-AP (VAV1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 16364-1-AP (same clone as 16364-1-PBS)

  IP result of anti-VAV1 (IP:16364-1-AP, 4ug; Detection:16364-1-AP 1:4000) with K-562 cells lysate 1800 ug. This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 16364-1-AP (same clone as 16364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 16364-1-AP (same clone as 16364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 16364-1-AP (same clone as 16364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 16364-1-AP (same clone as 16364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 16364-1-AP (same clone as 16364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 16364-1-AP (same clone as 16364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 16364-1-AP (same clone as 16364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 16364-1-AP (same clone as 16364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16364-1-AP (same clone as 16364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16364-1-AP (same clone as 16364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16364-1-AP (same clone as 16364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 16364-1-AP (same clone as 16364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 16364-1-AP (same clone as 16364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16364-1-AP (same clone as 16364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16364-1-AP (same clone as 16364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 16364-1-AP (same clone as 16364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 16364-1-AP (same clone as 16364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16364-1-PBS bindet in WB, IHC, IP, Indirect ELISA VAV1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VAV1 fusion protein Ag9049
• Vollständiger Name: vav 1 guanine nucleotide exchange factor
• Berechnetes Molekulargewicht: 845 aa, 98 kDa
• Beobachtetes Molekulargewicht: 98 kDa
• GenBank-Zugangsnummer: BC013361
• Gene symbol: VAV1
• Gene ID (NCBI): 7409
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vav proteins mainly act as enzymes that catalyze the activation step of Rho subfamily GTPases during cell signaling. There are three family members:VAV1, VAV2 and VAV3. Vav1 is specifically expressed in the hematopoietic system, whereas Vav2 and Vav3 are more ubiquitously expressed (PMID:14607270). Vav1 is physiologically active as a GDP/GTP nucleotide exchange factor (GEF) in the hematopoietic system (PMID:31654719).