VBP1 Polyklonaler Antikörper

VBP1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 14345-1-AP

Synonyme

von Hippel-Lindau-binding protein 1, VHL-binding protein 1, VBP-1, PFDN3, PFD3

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  WB analysis using 14345-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14345-1-AP (VBP1 antibody) at dilution of 1:8000 incubated at 4 degree celsius over night.

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  WB analysis of K-562 using 14345-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14345-1-AP (VBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14345-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14345-1-AP (VBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14345-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14345-1-AP (VBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 14345-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14345-1-AP (VBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 14345-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14345-1-AP (VBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14345-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using VBP1 antibody (14345-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen, HepG2-Zellen, K-562-Zellen, Maushirngewebe, Mauslebergewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

14345-1-AP bindet in WB, IHC, IF/ICC, ELISA VBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VBP1 fusion protein Ag5685
• Vollständiger Name: von Hippel-Lindau binding protein 1
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 23-25 kDa
• GenBank-Zugangsnummer: BC046094
• Gene symbol: VBP1
• Gene ID (NCBI): 7411
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	IF	PLoS Genet; The prefoldin complex stabilizes the von Hippel-Lindau protein against aggregation and degradation.; Authors - Franck Chesnel