VCAM-1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VCAM-1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

83

Kat-Nr. : 11444-1-AP

Synonyme

CD106, INCAM 100, V CAM 1, VCAM 1, VCAM1, VCAM-1

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Galerie der Validierungsdaten

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  WB analysis using 11444-1-AP

  Untreated or TNF-a-treated HUVEC cells were subjected to SDS PAGE followed by western blot with 11444-1-AP (VCAM-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11444-1-AP (VCAM-1 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11444-1-AP (VCAM-1 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: TNF alpha treated HUVEC cells
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lungenkarzinomgewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• IHC: See 21 publications below
• IF: See 19 publications below
• FC: See 1 publications below

Produktinformation

11444-1-AP bindet in WB, IHC, IF, FC, ELISA VCAM-1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VCAM-1 fusion protein Ag1993
• Vollständiger Name: vascular cell adhesion molecule 1
• Berechnetes Molekulargewicht: 739 aa, 81 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC017276
• Gene symbol: VCAM1
• Gene ID (NCBI): 7412
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

Publikationen

Species	Application	Title
	IF	Cardiovasc Res; HDAC4 regulates vascular inflammation via activation of autophagy.; Authors - Di Yang
human	IF	EBioMedicine; Interactions between neutrophil extracellular traps and activated platelets enhance procoagulant activity in acute stroke patients with ICA occlusion.; Authors - Peng Zhou
	IHC	Aging Dis; Sterol-resistant SCAP Overexpression in Vascular Smooth Muscle Cells Accelerates Atherosclerosis by Increasing Local Vascular Inflammation through Activation of the NLRP3 Inflammasome in Mice.; Authors - Danyang Li
human		Br J Pharmacol; Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.; Authors - Chuanrui Ma
	IF	Br J Pharmacol; Substitution of the SERCA2 Cys(674) reactive thiol accelerates atherosclerosis by inducing endoplasmic reticulum stress and inflammation; Authors - Hang Su
		Theranostics; Ligand Activation of PPARγ by Ligustrazine Suppresses Pericyte Functions of Hepatic Stellate Cells via SMRT-Mediated Transrepression of HIF-1α.; Authors - Feng Zhang

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