VCAM-1/CD106 Polyklonaler Antikörper

VCAM-1/CD106 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 30958-1-AP

Synonyme

CD106, VCAM-1, Vcam1, Vcam 1

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  WB analysis using 30958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30958-1-AP (VCAM-1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 30958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30958-1-AP (VCAM-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 30958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30958-1-AP (VCAM-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 30958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30958-1-AP (VCAM-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 30958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30958-1-AP (VCAM-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 30958-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using VCAM-1 antibody (30958-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 30958-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using VCAM-1 antibody (30958-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenmilzgewebe, C2C12-Zellen, Mausmilzgewebe
• Erfolgreiche Detektion in IHC: Mausmilzgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

30958-1-AP bindet in WB, IHC, IF-P, ELISA VCAM-1/CD106 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: vascular cell adhesion molecule 1
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: CAA47989.1
• Gene symbol: VCAM-1
• Gene ID (NCBI): 22329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

Publikationen

Species	Application	Title
mouse	IHC	Microvasc Res; Characterization of microcirculatory endothelial functions in a D-Galactose-induced aging model; Authors - Zhuo Li
mouse	IF	Redox Biol; Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation; Authors - Hui He