VCAM-1/CD106 Rekombinanter Antikörper

VCAM-1/CD106 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240597B2

Kat-Nr. : 83719-1-PBS

Synonyme

CD106, Vcam1, VCAM-1, 240597B2, Vcam 1

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  WB analysis of 3T3-L1 using 83719-1-RR (same clone as 83719-1-PBS)

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 83719-1-RR (VCAM-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83719-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP00701-4

  Sandwich ELISA standard curve of MP00701-4, Mouse VCAM-1/CD106 Recombinant Matched Antibody Pair - PBS only. 83719-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0639. 83719-3-PBS was HRP conjugated as the detection antibody. Range: 46.88-3000 pg/mL.

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  Sample test of MP00701-4

  Serum of eight mice was measured. The VCAM-1 concentration of detected samples was determined to be 572.76 ng/mL with a range of 483.18-844.96 ng/mL.

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  Sample test of MP00701-4

  Urine of eight mice was measured. The VCAM-1 concentration of detected samples was determined to be 71.97 ng/mL with a range of 8.65-105.58 ng/mL.

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  Sample test of MP00701-4

  Mouse lung, cut into 1-2 mm pieces, was cultured for 3 days in 25-30 mL of RPMI supplemented with 10% fetal bovine serum and unstimulated or stimulated with 10 μg/mL ConA. The mean VCAM-1 concentration was determined to be 14.2 ng/mL in unstimulated mouse lung superantant, 20.9 ng/mL in ConA stimulated mouse lung superantant.

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  IHC staining of mouse spleen using 83719-1-RR (same clone as 83719-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83719-1-RR (VCAM-1/CD106 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83719-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 83719-1-RR (same clone as 83719-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83719-1-RR (VCAM-1/CD106 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83719-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83719-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83719-1-RR against Mouse VCAM-1 were performed. The affinity constant is 0.383 nM.

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Geprüfte Anwendungen

Produktinformation

83719-1-PBS bindet in WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test VCAM-1/CD106 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: VCAM-1/CD106 fusion protein Eg0639
• Vollständiger Name: vascular cell adhesion molecule 1
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: CAA47989.1
• Gene symbol: VCAM-1
• Gene ID (NCBI): 22329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).