VCAM-1/CD106 Polyklonaler Antikörper

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• KD/KO Validated

VCAM-1/CD106 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11444-1-PBS

Synonyme

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  WB analysis using 11444-1-AP (same clone as 11444-1-PBS)

  Untreated or TNF-a-treated HUVEC cells were subjected to SDS PAGE followed by western blot with 11444-1-AP (VCAM-1/CD106 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 11444-1-AP (same clone as 11444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11444-1-AP (VCAM-1/CD106 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 11444-1-AP (same clone as 11444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11444-1-AP (VCAM-1/CD106 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 11444-1-AP (same clone as 11444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 11444-1-AP (same clone as 11444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 11444-1-AP (same clone as 11444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 11444-1-AP (same clone as 11444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 11444-1-AP (same clone as 11444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 11444-1-AP (same clone as 11444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 11444-1-AP (same clone as 11444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 11444-1-AP (same clone as 11444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 11444-1-AP (same clone as 11444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 11444-1-AP (same clone as 11444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11444-1-AP (same clone as 11444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11444-1-AP (same clone as 11444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11444-1-PBS bindet in WB, IHC, ELISA VCAM-1/CD106 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VCAM-1/CD106 fusion protein Ag1993
• Vollständiger Name: vascular cell adhesion molecule 1
• Berechnetes Molekulargewicht: 739 aa, 81 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC017276
• Gene symbol: VCAM-1
• Gene ID (NCBI): 7412
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).