VCAM-1/CD106 Rekombinanter Antikörper

VCAM-1/CD106 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241283G8

Kat-Nr. : 98079-1-PBS

Synonyme

VCAM1, VCAM-1, Soluble Vascular Cell Adhesion Molecule-1, L1CAM, INCAM-100

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  FC experiment of HUVEC using 98079-1-RR (same clone as 98079-1-PBS)

  1x10^6 TNF- α treated HUVEC cells were surface stained with 0.25 ug Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibody (98079-1-RR, Clone:241283G8)(red), or 0.25 ug Isotype Control (blue), or 1x10^6 untreated HUVEC cells were surface stained with 0.25 ug Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibody (98079-1-RR, Clone:241283G8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(black). Cells were not fixed. This data was developed using the same antibody clone with 98079-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HUVEC using 98079-1-RR (same clone as 98079-1-PBS)

  1x10^6 TNF- α treated HUVEC cells were surface stained with 0.25 ug Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibody (98079-1-RR, Clone:241283G8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98079-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HUVEC using 98079-1-RR (same clone as 98079-1-PBS)

  1x10^6 TNF- α treated HUVEC cells were surface stained with 0.25 ug Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibody (98079-1-RR, Clone:241283G8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98079-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98079-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98079-1-RR against Human VCAM-1/CD106 were performed. The affinity constant is 1.71 nM.

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Geprüfte Anwendungen

Produktinformation

98079-1-PBS bindet in FC VCAM-1/CD106 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: vascular cell adhesion molecule 1
• Berechnetes Molekulargewicht: 81 kDa
• GenBank-Zugangsnummer: NM_001078.4
• Gene symbol: VCAM-1
• Gene ID (NCBI): 7412
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).