VCAM-1/CD106 Rekombinanter Antikörper

VCAM-1/CD106 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241283G8

Kat-Nr. : 98079-1-RR

Synonyme

VCAM1, VCAM-1, Soluble Vascular Cell Adhesion Molecule-1, L1CAM, INCAM-100

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  FC experiment of HUVEC using 98079-1-RR

  1x10^6 TNF- α treated HUVEC cells were surface stained with 0.25 ug Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibody (98079-1-RR, Clone:241283G8)(red), or 0.25 ug Isotype Control (blue), or 1x10^6 untreated HUVEC cells were surface stained with 0.25 ug Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibody (98079-1-RR, Clone:241283G8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(black). Cells were not fixed.

• 

  FC experiment of HUVEC using 98079-1-RR

  1x10^6 TNF- α treated HUVEC cells were surface stained with 0.25 ug Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibody (98079-1-RR, Clone:241283G8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

• 

  FC experiment of HUVEC using 98079-1-RR

  1x10^6 TNF- α treated HUVEC cells were surface stained with 0.25 ug Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibody (98079-1-RR, Clone:241283G8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98079-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98079-1-RR against Human VCAM-1/CD106 were performed. The affinity constant is 1.71 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: TNF- α treated HUVEC cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98079-1-RR bindet in FC VCAM-1/CD106 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: vascular cell adhesion molecule 1
• Berechnetes Molekulargewicht: 81 kDa
• GenBank-Zugangsnummer: NM_001078.4
• Gene symbol: VCAM-1
• Gene ID (NCBI): 7412
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).