VCP Rekombinanter Antikörper

VCP Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3G23

Kat-Nr. : 82463-1-PBS

Synonyme

HEL-S-70, HEL-220, EC:3.6.4.6, 3G23, 15S Mg(2+)-ATPase p97 subunit

---

• 

  WB analysis using 82463-1-RR (same clone as 82463-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82463-1-RR (VCP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82463-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 82463-1-RR (same clone as 82463-1-PBS)

  IP result of anti-VCP (IP:82463-1-RR, 4ug; Detection:82463-1-RR 1:2000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 82463-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat small intestine using 82463-1-RR (same clone as 82463-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 82463-1-RR (VCP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82463-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 82463-1-RR (same clone as 82463-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using VCP antibody (82463-1-RR, Clone: 3G23 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82463-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82463-1-RR (same clone as 82463-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VCP antibody (82463-1-RR, Clone: 3G23 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82463-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82463-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82463-1-RR against Human VCP were performed. The affinity constant is 0.997 nM.

---

Geprüfte Anwendungen

Produktinformation

82463-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA VCP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: VCP fusion protein Ag1002
• Vollständiger Name: valosin-containing protein
• Berechnetes Molekulargewicht: 89 kDa
• Beobachtetes Molekulargewicht: 89 kDa
• GenBank-Zugangsnummer: BC007562
• Gene symbol: VCP
• Gene ID (NCBI): 7415
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).