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VDAC2 Polyklonaler Antikörper

VDAC2 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55261-1-AP

Synonyme

hVDAC2, VDAC 2, VDAC2

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of HepG2 cells using VDAC2 Polyclonal antibody (55261-1-AP)

    WB analysis of HepG2 using 55261-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55261-1-AP (VDAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using VDAC2 Polyclonal antibody (55261-1-AP)

    WB analysis of mouse brain using 55261-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55261-1-AP (VDAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HuH-7 cells using VDAC2 Polyclonal antibody (55261-1-AP)

    WB analysis of HuH-7 using 55261-1-AP

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 55261-1-AP (VDAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using VDAC2 Polyclonal antibody (55261-1-AP)

    IHC staining of human kidney using 55261-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using VDAC2 Polyclonal antibody (55261-1-AP)

    IHC staining of human kidney using 55261-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using VDAC2 Polyclonal antibody (55261-1-AP)

    IHC staining of human heart using 55261-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using VDAC2 Polyclonal antibody (55261-1-AP)

    IHC staining of human heart using 55261-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 40x lens).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, HuH-7-Zellen, Maushirngewebe
Erfolgreiche Detektion in IHChumanes Nierengewebe, humanes Herzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunhistochemie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

55261-1-AP bindet in WB, IHC, ELISA VDAC2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name voltage-dependent anion channel 2
Berechnetes Molekulargewicht 33 kDa
Beobachtetes Molekulargewicht 31-33 kDa
GenBank-ZugangsnummerNM_003375
Gene symbol VDAC2
Gene ID (NCBI) 7417
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20℃ lagern. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VDAC2 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. This antibody is specific to VDAC2.

Protokolle

Produktspezifische Protokolle
WB protocol for VDAC2 antibody 55261-1-APProtokoll herunterladen
IHC protocol for VDAC2 antibody 55261-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell Physiol Biochem

Knockout of VDAC1 in H9c2 Cells Promotes Oxidative Stress-Induced Cell Apoptosis through Decreased Mitochondrial Hexokinase II Binding and Enhanced Glycolytic Stress.

Authors - Meiying Yang
View Article