VDAC2 Monoklonaler Antikörper

VDAC2 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

1D8C11

Kat-Nr. : 66388-1-Ig

Synonyme

1D8C11, FLJ23841, hVDAC2, VDAC 2, VDAC-2

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  WB analysis of HEK-293 using 66388-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66388-1-Ig (VDAC2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 66388-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66388-1-Ig (VDAC2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 66388-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66388-1-Ig (VDAC2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 66388-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66388-1-Ig (VDAC2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 66388-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66388-1-Ig (VDAC2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 66388-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66388-1-Ig (VDAC2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 66388-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66388-1-Ig (VDAC2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 66388-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66388-1-Ig (VDAC2 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, ROS1728-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Nierengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

66388-1-Ig bindet in WB, IHC, ELISA VDAC2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: voltage-dependent anion channel 2
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 30-33 kDa
• GenBank-Zugangsnummer: NM_003375
• Gene symbol: VDAC2
• Gene ID (NCBI): 7417
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VDAC2 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules.

Publikationen

Species	Application	Title
human	WB	Cell Rep; Cardiolipin oxidized by ROS from complex II acts as a target of gasdermin D to drive mitochondrial pore and heart dysfunction in endotoxemia; Authors - Yan Tang
human	WB,IHC	Phytomedicine; Hedyotis diffusa injection induces ferroptosis via the Bax/Bcl2/VDAC2/3 axis in lung adenocarcinoma.; Authors - Fuhao Huang
human	WB	Drug Dev Res; Xanthatin inhibits non-small cell lung cancer proliferation by breaking the redox balance.; Authors - Yanbo Xie
human	WB	Cancer Discov; BRCA1-mediated dual regulation of ferroptosis exposes a vulnerability to GPX4 and PARP co-inhibition in BRCA1-deficient cancers; Authors - Guang Lei