VDAC2 Rekombinanter Antikörper

VDAC2 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241569G11

Kat-Nr. : 84225-2-PBS

Synonyme

241569G11, FLJ23841, hVDAC2, VDAC 2, VDAC-2

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  WB analysis using 84225-2-RR (same clone as 84225-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84225-2-RR (VDAC2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84225-2-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 84225-2-RR (same clone as 84225-2-PBS)

  IP result of anti-VDAC2 (IP:84225-2-RR, 4ug; Detection:84225-2-RR 1:2000) with HEK-293 cells lysate 1800 ug. This data was developed using the same antibody clone with 84225-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 84225-2-RR (same clone as 84225-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84225-2-RR (VDAC2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84225-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 84225-2-RR (same clone as 84225-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84225-2-RR (VDAC2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84225-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84225-2-RR (same clone as 84225-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VDAC2 antibody (84225-2-RR, Clone: 241569G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84225-2-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 84225-2-RR (same clone as 84225-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Vdac2 Recombinant Antibody (84225-2-RR, Clone:241569G11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84225-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01162-1

  Cytometric bead array standard curve of MP01162-1, VDAC2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84225-2-PBS. Detection antibody: 84225-1-PBS. Standard: Ag2266. Range: 1.563-200 ng/mL.

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  Affinity and Kinetic Characterization of 84225-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84225-2-RR against Human VDAC2 were performed. The affinity constant is 0.48 nM.

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Geprüfte Anwendungen

Produktinformation

84225-2-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA VDAC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: VDAC2 fusion protein Ag2266
• Vollständiger Name: voltage-dependent anion channel 2
• Berechnetes Molekulargewicht: 294 aa, 32 kDa
• Beobachtetes Molekulargewicht: 31-33 kDa
• GenBank-Zugangsnummer: BC000165
• Gene symbol: VDAC2
• Gene ID (NCBI): 7417
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VDACs (Voltage Dependent Anion selective Channels), also known as mitochondrial porins, are a family of pore-forming proteins discovered in the mitochondrial outer membrane. Mammals show a conserved genetic organization of the VDAC genes. It's reported that the amount of VDAC transcripts in liver is usually lower than in the other tissues. VDAC2 and especially VDAC3 are highly expressed in testis, while mouse VDAC1 is poorly expressed in this tissue. (PMID: 22020053)