VDAC3 Polyklonaler Antikörper

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• KD/KO Validated

VDAC3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Kat-Nr. : 55260-1-AP

Synonyme

HD VDAC3, hVDAC3, VDAC 3, VDAC-3

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  WB analysis of A431 using 55260-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55260-1-AP (VDAC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 55260-1-AP

  WB result of VDAC3 antibody (55260-1-AP; 1:400; room temperature for 1.5 hours) with wild-type and VDAC3 knockout HEK-293T cells.

• 

  WB analysis of mouse testis using 55260-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55260-1-AP (VDAC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 55260-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55260-1-AP (VDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293T-Zellen, Maushodengewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

55260-1-AP bindet in WB, IHC, IF, CoIP, ELISA VDAC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: voltage-dependent anion channel 3
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: NM_005662
• Gene symbol: VDAC3
• Gene ID (NCBI): 7419
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VDAC3 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. This antibody is specific to VDAC3.

Publikationen

Species	Application	Title
	WB	Nat Commun; VDAC2 enables BAX to mediate apoptosis and limit tumor development.; Authors - Hui San Chin
human	WB	Cell Rep; Cardiolipin oxidized by ROS from complex II acts as a target of gasdermin D to drive mitochondrial pore and heart dysfunction in endotoxemia; Authors - Yan Tang
human	WB	Cell Biosci; The genomic signature of resistance to platinum-containing neoadjuvant therapy based on single-cell data; Authors - Qihai Sui ; KD Validated
human	WB	Ecotoxicol Environ Saf; Lysosomal iron accumulation and subsequent lysosomes-mitochondria iron transmission mediate PFOS-induced hepatocyte ferroptosis; Authors - Jixun Li
human	WB	Front Cell Dev Biol; Autophagy Regulates VDAC3 Ubiquitination by FBXW7 to Promote Erastin-Induced Ferroptosis in Acute Lymphoblastic Leukemia.; Authors - Ting Zhu
human	WB,IHC	Int J Biol Sci; The lncRNA BDNF-AS/WDR5/FBXW7 axis mediates ferroptosis in gastric cancer peritoneal metastasis by regulating VDAC3 ubiquitination.; Authors - Guoquan Huang