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VE-cadherin/CD144 Rekombinanter Antikörper

VE-cadherin/CD144 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240755E10

Kat-Nr. : 83766-5-PBS

Synonyme

VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen

Filter:
  • Western Blot (WB) analysis of various lysates using VE-cadherin/CD144 Recombinant antibody (83766-5-RR)

    WB analysis using 83766-5-RR (same clone as 83766-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83766-5-RR (VE-cadherin/CD144 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83766-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83766-5-RR

    Affinity and Kinetic Characterization of 83766-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83766-5-RR against Human VE-cadherin were performed. The affinity constant is 51.2 nM.

Geprüfte Anwendungen

Produktinformation

83766-5-PBS bindet in WB, ELISA VE-cadherin/CD144 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Fusionsprotein
Vollständiger Name cadherin 5, type 2 (vascular endothelium)
Berechnetes Molekulargewicht88 kDa
Beobachtetes Molekulargewicht130 kDa
GenBank-ZugangsnummerNM_001795.5
Gene symbol VE-cadherin
Gene ID (NCBI) 1003
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).