VE-cadherin/CD144 Rekombinanter Antikörper

VE-cadherin/CD144 Rekombinant Antikörper für IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241953H6

Kat-Nr. : 84529-3-PBS

Synonyme

VE-cadherin, 241953H6, 7B4, cadherin 5, Cd144

---

• 

  Sandwich ELISA standard curve of MP01386-3

  Sandwich ELISA standard curve of MP01386-3, Mouse VE-cadherin/CD144 Recombinant Matched Antibody Pair - PBS only. 84529-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0921. 84529-6-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL.

• 

  Sample test of MP01386-3

  The mean VE-cadherin/CD144 concentration was determined to be 3,858.35 ng/mL in mouse heart tissue extract based on a 1.20 mg/mL extract load and 709.13 ng/mL in bEnd.3 cell extract based on a 1.80 mg/mL extract load.

• 

  IHC staining of mouse spleen using 84529-3-RR (same clone as 84529-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84529-3-RR (VE-cadherin/CD144 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84529-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84529-3-RR (same clone as 84529-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84529-3-RR (VE-cadherin/CD144 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84529-3-PBS in a different storage buffer formulation.

• 

  IF Staining of bEnd.3 using 84529-3-RR (same clone as 84529-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using VE-cadherin antibody (84529-3-RR, Clone: 241953H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84529-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84529-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84529-3-RR against Mouse VE-cadherin were performed. The affinity constant is 1.22 nM.

---

Geprüfte Anwendungen

Produktinformation

84529-3-PBS bindet in IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test VE-cadherin/CD144 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: VE-cadherin/CD144 fusion protein Eg0921
• Vollständiger Name: cadherin 5
• Berechnetes Molekulargewicht: 88kDa
• GenBank-Zugangsnummer: NM_009868.4
• Gene symbol: Cdh5
• Gene ID (NCBI): 12562
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609).