VE-cadherin Monoklonaler Antikörper

VE-cadherin Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

4F9B10

Kat-Nr. : CL594-66804

Synonyme

7B4, 7B4 antigen, Cadherin 5, CD144, CDH5, FLJ17376, Vascular endothelial cadherin, VE cadherin, VE-cadherin

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Galerie der Validierungsdaten

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  IF Staining of human breast cancer using CL594-66804

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 VE-cadherin antibody (CL594-66804, Clone: 4F9B10 ) at dilution of 1:100.

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  IF Staining of human tonsillitis using CL594-66804

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 VE-cadherin antibody (CL594-66804, Clone: 4F9B10 ) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Mammakarzinomgewebe, humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66804 bindet in IF VE-cadherin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VE-cadherin fusion protein Ag27501
• Vollständiger Name: cadherin 5, type 2 (vascular endothelium)
• Berechnetes Molekulargewicht: 88 kDa
• GenBank-Zugangsnummer: NM_001795
• Gene symbol: CDH5
• Gene ID (NCBI): 1003
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). E-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609).