VE-cadherin/CD144 Rekombinanter Antikörper

VE-cadherin/CD144 Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

240755D5

Kat-Nr. : 83766-6-RR

Synonyme

VE-cadherin, 240755D5, 7B4, 7B4 antigen, Cadherin 5

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  WB analysis using 83766-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83766-6-RR (VE-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 83766-6-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83766-6-RR (VE-cadherin/CD144 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 83766-6-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83766-6-RR (VE-cadherin/CD144 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 83766-6-RR

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using VE-cadherin/CD144 antibody (83766-6-RR, Clone: 240755D5 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HUVEC using 83766-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using VE-cadherin/CD144 antibody (83766-6-RR, Clone: 240755D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83766-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83766-6-RR against Human VE-cadherin/CD144 were performed. The affinity constant is 0.633 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IF/ICC: HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)-P: IF-P : 1:150-1:600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

83766-6-RR bindet in WB, IHC, IF/ICC, IF-P, ELISA VE-cadherin/CD144 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: VE-cadherin/CD144 fusion protein Eg1200
• Vollständiger Name: cadherin 5, type 2 (vascular endothelium)
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa, 130-140 kDa
• GenBank-Zugangsnummer: NM_001795.5
• Gene symbol: VE-cadherin
• Gene ID (NCBI): 1003
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).

Publikationen

Species	Application	Title
human	WB,IF	J Cell Mol Med; Single-Cell Transcriptomic Reveals the Involvement of Cell-Cell Junctions in the Early Development of Hypertrophic Cardiomyopathy; Authors - Dingchen Wang