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VE-cadherin/CD144 Rekombinanter Antikörper

VE-cadherin/CD144 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241953A12

Kat-Nr. : 84529-4-PBS

Synonyme

VE-cadherin, VE Cad, Cdh5, Cd144, cadherin 5

Filter:
  • Western Blot (WB) analysis of various lysates using VE-cadherin/CD144 Recombinant antibody (84529-4-RR)

    WB analysis using 84529-4-RR (same clone as 84529-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84529-4-RR (VE-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84529-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84529-4-RR

    Affinity and Kinetic Characterization of 84529-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84529-4-RR against Mouse VE-cadherin/CD144 were performed. The affinity constant is 51.6 nM.

Geprüfte Anwendungen

Produktinformation

84529-4-PBS bindet in WB, Indirect ELISA VE-cadherin/CD144 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name cadherin 5
Berechnetes Molekulargewicht88kDa
Beobachtetes Molekulargewicht125-130 kDa, 90-100 kDa
GenBank-ZugangsnummerNM_009868.4
Gene symbol Cdh5
Gene ID (NCBI) 12562
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).