VE-cadherin/CD144 Rekombinanter Antikörper

VE-cadherin/CD144 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241953A12

Kat-Nr. : 84529-4-RR

Synonyme

VE-cadherin, VE Cad, Cdh5, Cd144, cadherin 5

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  WB analysis using 84529-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84529-4-RR (VE-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84529-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84529-4-RR against Mouse VE-cadherin/CD144 were performed. The affinity constant is 51.6 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: bEnd.3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84529-4-RR bindet in WB, ELISA VE-cadherin/CD144 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: cadherin 5
• Berechnetes Molekulargewicht: 88kDa
• Beobachtetes Molekulargewicht: 125-130 kDa, 90-100 kDa
• GenBank-Zugangsnummer: NM_009868.4
• Gene symbol: Cdh5
• Gene ID (NCBI): 12562
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).