VE-cadherin/CD144 Rekombinanter Antikörper

VE-cadherin/CD144 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241953D4

Kat-Nr. : 98251-1-RR

Synonyme

VE-cadherin, VE Cad, Cdh5, Cd144, cadherin 5

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  FC experiment of bEnd.3 using 98251-1-RR

  1x10^6 bEnd.3 cells (left) or C2C12 (right) were surface stained with 0.25 ug Anti-Mouse VE-cadherin/CD144 Rabbit Recombinant Antibody (98251-1-RR, Clone: 241953D4) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98251-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98251-1-RR against Mouse VE-cadherin/CD144 were performed. The affinity constant is 1.65 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: bEnd.3-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98251-1-RR bindet in FC VE-cadherin/CD144 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: cadherin 5
• Berechnetes Molekulargewicht: 88kDa
• GenBank-Zugangsnummer: NM_009868.4
• Gene symbol: Cdh5
• Gene ID (NCBI): 12562
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609).