VE-cadherin/CD144 Rekombinanter Antikörper

VE-cadherin/CD144 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

240755B2

Kat-Nr. : PE-FcA98071

Synonyme

VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen

---

• 

  FC experiment of HUVEC using PE-FcA98071

  1x10^6 HUVEC cells were surface stained with 5 ul PE Anti-Human VE-cadherin/CD144 Rabbit RecAb (PE-FcA98071, Clone:240755B2) (red) or PE Rabbit IgG Isotype Control RecAb (PE-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: HUVEC-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-FcA98071 bindet in FC VE-cadherin/CD144 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: cadherin 5, type 2 (vascular endothelium)
• Berechnetes Molekulargewicht: 88 kDa
• GenBank-Zugangsnummer: NM_001795.5
• Gene symbol: VE-cadherin
• Gene ID (NCBI): 1003
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).