VEGFA Rekombinanter Antikörper

VEGFA Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240573B3

Kat-Nr. : 83610-2-PBS

Synonyme

Vegf, VEGF164

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  Sandwich ELISA standard curve of MP00598-3

  Sandwich ELISA standard curve of MP00598-3, Rat VEGF Recombinant Matched Antibody Pair - PBS only. 83610-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0941. 83610-2-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

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  Sample test of MP00598-3

  Rat lung, cut into 1-2 mm pieces, was cultured for 4 days in 25-30 mL of RPMI supplemented with 10% fetal bovine serum and stimulated with 2.5 ng/mL LPS. The cell culture supernatant was removed, assayed for rat VEGF, and measured 3292.9 pg/mL. Rat spleen, cut into 1-2 mm pieces, was cultured for 3-4 days in 25-30 mL of RPMI supplemented with 10% fetal bovine serum and stimulated with 10 μg/mL ConA. The cell culture supernatant was removed, assayed for rat VEGF, and measured 433.1 pg/mL.

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  Sample test of MP00598-3

  Serum of eight rats was measured. The VEGF concentration of one sample was determined to be 34.3 pg/mL, and the range of VEGF 164 concentration was 0 - 34.3 pg/mL.

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  Affinity and Kinetic Characterization of 83610-2-PBS

  Biolayer interferometry (BLl) kinetic assays of 84610-2-PBS against Rat VEGFA were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83610-2-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test VEGFA und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: VEGFA fusion protein Eg0941
• Vollständiger Name: vascular endothelial growth factor A
• Berechnetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: NM_001287108.1
• Gene symbol: Vegfa
• Gene ID (NCBI): 83785
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.