VEGFA Rekombinanter Antikörper

VEGFA Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242072G9

Kat-Nr. : 84687-2-PBS

Synonyme

VEGF, 242072G9, L-VEGF, N-VEGF, Vascular Endothelial Growth Factor

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  WB analysis of Recombinant protein using 84687-2-RR (same clone as 84687-2-PBS)

  Recombinant protein VEGF121 (Eg1263) was subjected to SDS PAGE followed by western blot with 84687-2-RR (VEGFA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84687-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01495-1

  Cytometric bead array standard curve of MP01495-1, VEGF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84687-1-PBS. Detection antibody: 84687-2-PBS. Standard: Eg1263. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84687-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 84687-2-PBS against Human VEGF121 was performed. The affinity constant is 0.882 nM.

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  Affinity and Kinetic Characterization of 84687-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84687-2-RR against Human VEGFA were performed. The affinity constant is 92.0 pM.

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Geprüfte Anwendungen

Produktinformation

84687-2-PBS bindet in WB, Cytometric bead array, Indirect ELISA VEGFA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: VEGFA fusion protein Eg1263
• Vollständiger Name: vascular endothelial growth factor A
• GenBank-Zugangsnummer: NM_001171628.1
• Gene symbol: VEGFA
• Gene ID (NCBI): 7422
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VEGFA, also named VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).