VEGFA Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VEGFA Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (5)

Anwendung

WB, RIP, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

635

Rezensionen (2)

Kat-Nr. : 19003-1-AP

Synonyme

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF

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Galerie der Validierungsdaten

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  WB analysis using 19003-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19003-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 19003-1-AP

  IP result of anti-VEGFA(IP:19003-1-AP, 4ug; Detection:19003-1-AP 1:3000) with Jurkat cells lysate 1560 ug.

• 

  IHC staining of human lung cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of NIH/3T3 using 19003-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human VEGFA (19003-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Leberkarzinomgewebe, Mauslebergewebe, Mauslungengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 11 publications below
• IHC: See 195 publications below
• IF: See 61 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

19003-1-AP bindet in WB, RIP, IP, IHC, IF, FC, ELISA VEGFA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Kaninchen, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VEGFA fusion protein Ag13500
• Vollständiger Name: vascular endothelial growth factor A
• Berechnetes Molekulargewicht: 412 aa, 46 kDa
• Beobachtetes Molekulargewicht: 36-40 kDa
• GenBank-Zugangsnummer: BC065522
• Gene symbol: VEGFA
• Gene ID (NCBI): 7422
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ). This antibody can recognize all VEGFA isoforms.

Publikationen

Species	Application	Title
mouse	IHC	Nat Nanotechnol; Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades.; Authors - Xuefei Lu ; KD Validated
mouse,human		ACS Nano; Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.; Authors - Meng Zhao
mouse	IHC	Biomaterials; A nano-conductive osteogenic hydrogel to locally promote calcium influx for electro-inspired bone defect regeneration; Authors - Congcong Yu
mouse		Light Sci Appl; Microglia modulation with 1070-nm light attenuates Aβ burden and cognitive impairment in Alzheimer's disease mouse model.; Authors - Lechan Tao
human	IP	J Clin Invest; Angiopoietin-like 4 binds neuropilins and cooperates with VEGF to induce diabetic macular edema.; Authors - Akrit Sodhi
human		Bone Res; Endothelial PDGF-BB/PDGFR-β signaling promotes osteoarthritis by enhancing angiogenesis-dependent abnormal subchondral bone formation; Authors - Zhuang Cui

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alexandru (Verified Customer) (11-21-2023)

Great antibody with clear and specific signal, would buy again!

• Applications: Western Blot
• Cell Tissue Type: Mouse Ventricle

FH

Fiona (Verified Customer) (07-16-2019)

Good quality antibody and good value for money. Would purchase again.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: ARPE-19

VEGFA Antibody Western Blot, validation (1:500 dilution) in ARPE-19 (Cat no:19003-1-AP)

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