VEGFA Rekombinanter Antikörper

VEGFA Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241391G1

Kat-Nr. : 84093-2-PBS

Synonyme

L-VEGF , N-VEGF, Vascular Endothelial Growth Factor, vascular endothelial growth factor A, Vascular endothelial growth factor A, long form

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  Sandwich ELISA standard curve of MP00561-4

  Sandwich ELISA standard curve of MP00561-4, Mouse VEGF Recombinant Matched Antibody Pair - PBS only. 84093-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0278. 84093-5-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL

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  Sample test of MP00561-4

  Serum of eight mice was measured. The mouse VEGF concentration of detected samples was determined to be 12.6 pg/mL with a range of 7.3 - 19.7 pg/mL.

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  Sample test of MP00561-4

  Mouse lung tissue was rinsed with 1X PBS to remove excess blood, homogenized in 20 mL of 1X PBS and stored overnight at ≤ -20 °C. Homogenates were thawed and centrifuged for 5 minutes at 5000 x g. Homogenates were assayed for mouse VEGF and measured 775.6 pg/mL based on a 1.8 mg/mL extract load.

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  Affinity and Kinetic Characterization of 84093-2-PBS

  Biolayer interferometry (BLl) kinetic assays of 84093-2-PBS against Mouse VEGFA were performed. The affinity constant is 0.859 nM.

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Geprüfte Anwendungen

Produktinformation

84093-2-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test VEGFA und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: VEGFA fusion protein Eg0278
• Vollständiger Name: vascular endothelial growth factor A
• Berechnetes Molekulargewicht: 17kd
• GenBank-Zugangsnummer: NM_001287058
• Gene symbol: Vegfa
• Gene ID (NCBI): 22339
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.