VEGFB Rekombinanter Antikörper

VEGFB Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242024C10

Kat-Nr. : 84734-3-RR

Synonyme

242024C10, VEGF B, VEGF related factor, VEGF-B, VEGF-related factor

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  WB analysis of Transfected HEK-293T using 84734-3-RR

  Human VEGFB Transfected HEK-293T cells were subjected to SDS PAGE followed by western blot with 84734-3-RR (VEGFB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Transfected CHO-K1 using 84734-3-RR

  Immunofluorescent analysis of (4% PFA) fixed Transfected CHO-K1 cells using VEGFB antibody (84734-3-RR, Clone: 242024C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84734-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84734-3-RR against Human VEGFB were performed. The affinity constant is 4.28 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Human VEGFB Transfected HEK-293T cells
• Erfolgreiche Detektion in IF/ICC: Transfected CHO-K1

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84734-3-RR bindet in WB, IF/ICC, ELISA VEGFB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: VEGFB fusion protein Eg2458
• Vollständiger Name: vascular endothelial growth factor B
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22-30 kDa
• GenBank-Zugangsnummer: BC008818
• Gene symbol: VEGFB
• Gene ID (NCBI): 7423
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vascular endothelial growth factor B (VEGFB) is the third vascular endothelial growth factor family member to be discovered in 1996 . It has been demonstrated that vascular endothelial growth factor B (VEGFB) plays a vital role in regulating vascular biological function. VEGFB binds to vascular endothelial growth factor receptor 1 (VEGFR1) and neuropilin 1 (NRP1), which activates a lot of downstream activators similar to most tyrosine kinase receptors such as p38 MAPK, ERK/MAPK, PKB/AKT, and PI3K.(PMID: 34948148, PMID: 37375853)