VEGFR2/KDR Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VEGFR2/KDR Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(135)

Rezensionen (3)

Kat-Nr. : 26415-1-AP

Synonyme

KDR, VEGFR2, Vascular endothelial growth factor receptor 2, Protein-tyrosine kinase receptor flk-1, FLK-1

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  WB analysis using 26415-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26415-1-AP (VEGFR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 26415-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 26415-1-AP (VEGF Receptor 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 26415-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26415-1-AP (VEGF Receptor 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 26415-1-AP

  IP result of anti-VEGFR2/KDR (IP:26415-1-AP, 4ug; Detection:26415-1-AP 1:3000) with human placenta tissue lysate 600 ug.

• 

  IP experiment of rat skeletal muscle using 26415-1-AP

  IP result of anti-VEGFR2/KDR (IP:26415-1-AP, 4ug; Detection:26415-1-AP 1:1000) with rat skeletal muscle tissue lysate 760 ug.

• 

  IHC staining of human placenta using 26415-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26415-1-AP (VEGFR2/KDR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 26415-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26415-1-AP (VEGFR2/KDR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, Mausherzgewebe, Mauslungengewebe, Maus-Plazenta-Gewebe, Rattenlungengewebe
• Erfolgreiche IP: humanes Plazenta-Gewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 99 publications below
• IHC: See 19 publications below
• IF: See 37 publications below
• IP: See 2 publications below
• FC: See 3 publications below
• CoIP: See 2 publications below

Produktinformation

26415-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA VEGFR2/KDR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VEGFR2/KDR fusion protein Ag24589
• Vollständiger Name: kinase insert domain receptor (a type III receptor tyrosine kinase)
• Berechnetes Molekulargewicht: 1356 aa, 152 kDa
• Beobachtetes Molekulargewicht: 150 kDa, 230 kDa
• GenBank-Zugangsnummer: BC131822
• Gene symbol: KDR
• Gene ID (NCBI): 3791
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KDR, also named as VEGFR-2, FLK1 and CD309, is a receptor for VEGF or VEGFC. KDR which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.

Publikationen

Species	Application	Title
human	WB	Bioact Mater; A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds; Authors - Shunlai Shang
human	IF	Sci Bull (Beijing); Restoring sweat gland function in mice using regenerative sweat gland cells derived from chemically reprogrammed human epidermal keratinocytes; Authors - Jiangbing Xiang
mouse	WB	J Exp Med; Secretogranin III as a disease-associated ligand for antiangiogenic therapy of diabetic retinopathy.; Authors - Michelle E LeBlanc
human	WB,IHC	Aging Dis; MSC-Derived Exosomes can Enhance the Angiogenesis of Human Brain MECs and Show Therapeutic Potential in a Mouse Model of Parkinson's Disease.; Authors - Chunling Xue
	WB,IF	J Neuroinflammation; Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization; Authors - Tianyi Shen
human	WB	Bioact Mater; Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid.; Authors - Wenjing Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Poulomi (Verified Customer) (07-02-2024)

The blot was good. It detected VEGFR2 in brain endothelial cells and Hek cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Endothelial Cells

FH

Sammy (Verified Customer) (01-20-2024)

This works well to detect VEGFR2 in HUVEC lysate. Ab diluted in 3% BSA PBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HUVEC

FH

Kyosuke (Verified Customer) (06-12-2019)

I am working on angiogenesis study and use this for WB on HUVECs. It works very well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HUVECs