VEZT Polyklonaler Antikörper

VEZT Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17684-1-PBS

Synonyme

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  WB analysis using 17684-1-AP (same clone as 17684-1-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 17684-1-AP (VEZT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 17684-1-AP (same clone as 17684-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 17684-1-AP (VEZT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 17684-1-AP (same clone as 17684-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17684-1-AP (VEZT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 17684-1-AP (same clone as 17684-1-PBS)

  IP result of anti-VEZT (IP:17684-1-AP, 4ug; Detection:17684-1-AP 1:500) with mouse brain tissue lysate 4400ug. This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17684-1-AP (same clone as 17684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17684-1-AP (VEZT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 17684-1-AP (same clone as 17684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17684-1-AP (VEZT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17684-1-PBS bindet in WB, IHC, IP, Indirect ELISA VEZT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VEZT fusion protein Ag11786
• Vollständiger Name: vezatin, adherens junctions transmembrane protein
• Berechnetes Molekulargewicht: 731 aa, 83 kDa
• Beobachtetes Molekulargewicht: 89 kDa, 125 kDa
• GenBank-Zugangsnummer: BC064939
• Gene symbol: VEZT
• Gene ID (NCBI): 55591
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vezatin (VEZT) is an adherens junctions transmembrane protein which identified as a putative tumor suppressor. In case of Listeria infection, it promotes bacterial internalization by participating in myosin VIIa recruitment to the entry site. There're two isoforms with calculated MW 89 kDa and 65 kDa. With modification or glycosylation, the MW in Western blot detection will be presented as ~125 kDa and ~89 kDa (PMID: 11080149) or 65-71 kDa and 88-100 kDa (PMID: 20049712). There're some isoforms with MW less than 70 kDa.