SLC32A1/VGAT Polyklonaler Antikörper

SLC32A1/VGAT Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14471-1-PBS

Synonyme

SLC32A1, VGAT, GABA and glycine transporter, hVIAAT, Vesicular GABA transporter

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  WB analysis of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

  unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 14471-1-AP (VGAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

  IP result of anti-VGAT (IP:14471-1-AP, 4ug; Detection:14471-1-AP 1:5000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14471-1-AP (VGAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14471-1-AP (VGAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using SLC32A1/VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14471-1-PBS bindet in WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA SLC32A1/VGAT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC32A1/VGAT fusion protein Ag5843
• Vollständiger Name: solute carrier family 32 (GABA vesicular transporter), member 1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC053582
• Gene symbol: VGAT
• Gene ID (NCBI): 140679
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC32A1, also known as VGAT (vesicular GABA transporter), functions in the uptake of GABA and glycine into synaptic vesicles. GABA (gamma-aminobutyric acid), is the major inhibitory neurotransmitter in the CNS. VGAT transports GABA and glycine into acidic vesicles and localizes to the synaptic vesicle in glycinergic and GABAergic neurons. And VGAT antibodies are useful markers for presynaptic GABAergic and glycinergic neurons.