VGF Polyklonaler Antikörper

VGF Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 26781-1-AP

Synonyme

SCG7, Neurosecretory protein VGF, Neuro-Endocrine Specific Protein VGF, Neuroendocrine regulatory peptide-2, Neuroendocrine regulatory peptide-1

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  WB analysis using 26781-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26781-1-AP (VGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of U2OS using 26781-1-AP

  U2OS lysates prepared and IP of VGF performed using 1.0 μg of 26781-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human brain using 26781-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26781-1-AP (VGF Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 26781-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26781-1-AP (VGF Antibody) at dilution of 1:200 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Maushirngewebe
• Erfolgreiche IP: U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

26781-1-AP bindet in WB, IHC, IF, IP, ELISA VGF und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VGF fusion protein Ag24820
• Vollständiger Name: VGF nerve growth factor inducible
• Berechnetes Molekulargewicht: 615 aa, 67 kDa
• Beobachtetes Molekulargewicht: 67 kDa, 90-100 kDa
• GenBank-Zugangsnummer: BC063835
• Gene symbol: VGF
• Gene ID (NCBI): 7425
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VGF encodes a neuropeptide precursor with specific expression in a subpopulation of neuroendocrine cells, and it is upregulated by nerve growth factor. The expression of VGF is developmentally regulated in different areas of the brain (PubMed: 18406753). Exercise upregulated expression of VGF in mouse hippocampus, and a synthetic Vgf-derived peptide produced an antidepressant response in mice (PubMed: 18059283). VGF can be detected the precursor form of 90-100 kDa before proteolytic cleavage (PMID:24934694, PMID:10433233).

Publikationen

Species	Application	Title
human	IF	Mol Biol Cell; Microtubule dynamics influence the retrograde biased motility of kinesin-4 motor teams in neuronal dendrites.; Authors - Erin M Masucci
human	WB	Clin Epigenetics; METTL3 exerts synergistic effects on m6A methylation and histone modification to regulate the function of VGF in lung adenocarcinoma; Authors - Kesong Shi
human	WB,IHC	J Exp Clin Cancer Res; Hedgehog-Gli1-derived exosomal circ-0011536 mediates peripheral neural remodeling in pancreatic cancer by modulating the miR-451a/VGF axis; Authors - Weiqi Dai

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

James (Verified Customer) (09-15-2020)

Antibody works well, it is able to detect both GST-tagged VGF expressed in HeLa cell lysates, and HRV 3C protease cleaved VGF after IVT reaction and GST purification.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cell lysate IVT expression

VGF Antibody Western Blot validation (1:1000 dilution) in HeLa cell lysate IVT expression (Cat no:26781-1-AP)