VGF Polyklonaler Antikörper

VGF Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26781-1-PBS

Synonyme

SCG7, Neurosecretory protein VGF, Neuro-Endocrine Specific Protein VGF, Neuroendocrine regulatory peptide-2, Neuroendocrine regulatory peptide-1

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  WB analysis using 26781-1-AP (same clone as 26781-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26781-1-AP (VGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26781-1-PBS in a different storage buffer formulation.

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  IP experiment of U2OS using 26781-1-AP (same clone as 26781-1-PBS)

  U2OS lysates prepared and IP of VGF performed using 1.0 μg of 26781-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 26781-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 26781-1-AP (same clone as 26781-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26781-1-AP (VGF Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 26781-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 26781-1-AP (same clone as 26781-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26781-1-AP (VGF Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 26781-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

26781-1-PBS bindet in WB, IHC, IP, Indirect ELISA VGF und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VGF fusion protein Ag24820
• Vollständiger Name: VGF nerve growth factor inducible
• Berechnetes Molekulargewicht: 615 aa, 67 kDa
• Beobachtetes Molekulargewicht: 67 kDa, 90-100 kDa
• GenBank-Zugangsnummer: BC063835
• Gene symbol: VGF
• Gene ID (NCBI): 7425
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VGF encodes a neuropeptide precursor with specific expression in a subpopulation of neuroendocrine cells, and it is upregulated by nerve growth factor. The expression of VGF is developmentally regulated in different areas of the brain (PubMed: 18406753). Exercise upregulated expression of VGF in mouse hippocampus, and a synthetic Vgf-derived peptide produced an antidepressant response in mice (PubMed: 18059283). VGF can be detected the precursor form of 90-100 kDa before proteolytic cleavage (PMID:24934694, PMID:10433233).