VGLL1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VGLL1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 10124-2-AP

Synonyme

Protein TONDU, TDU, vestigial like 1 (Drosophila), Vgl 1, VGL1, VGLL1

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Galerie der Validierungsdaten

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  WB analysis using 10124-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10124-2-AP (VGLL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10124-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10124-2-AP (VGLL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10124-2-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10124-2-AP (VGLL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10124-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10124-2-AP (VGLL1 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, humanes Hirngewebe, Maushirngewebe, Mausmilzgewebe, PC-3-Zellen, Rattenhirngewebe, Rattenmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

10124-2-AP bindet in WB, IHC, ELISA VGLL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VGLL1 fusion protein Ag0169
• Vollständiger Name: vestigial like 1 (Drosophila)
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 26-29 kDa
• GenBank-Zugangsnummer: BC000045
• Gene symbol: VGLL1
• Gene ID (NCBI): 51442
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VGLL1 is a specific coactivator for the mammalian TEFs. It can bind proteins of the TEA domain family of transcription factors. Vgll1-TEFs complex upregulates the expression of IGFBP-5, a proliferation-promoting gene, and facilitates anchorage-independent cell proliferation. The calculated molecular weight of VGLL1 is 29 kDa, and the observed molecular weight is 26-29 kDa(PMID:31816819,33087697).

Publikationen

Species	Application	Title
		Endocr Relat Cancer; Vgll1 expression is associated with a triple-negative basal-like phenotype in breast cancer.; Authors - María Ángeles Castilla
human	WB,IHC	Cancers (Basel); PI3K/AKT/β-Catenin Signaling Regulates Vestigial-Like 1 Which Predicts Poor Prognosis and Enhances Malignant Phenotype in Gastric Cancer.; Authors - Bo-Kyung Kim ; KD Validated
human	WB	Int J Mol Sci; TNF−α Secreted from Macrophages Increases the Expression of Prometastatic Integrin αV in Gastric Cancer; Authors - Mi-Aie Hwang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jennifer (Verified Customer) (06-10-2022)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human urothelial cells