VHL Polyklonaler Antikörper

VHL Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (3)

Kat-Nr. : 16538-1-AP

Synonyme

HRCA1, Protein G7, pVHL, RCA1, VHL, VHL1, Von Hippe-Lindau

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Galerie der Validierungsdaten

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  IHC staining of human liver cancer using 16538-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16538-1-AP (VHL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16538-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16538-1-AP (VHL antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16538-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16538-1-AP (VHL antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 16538-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16538-1-AP (VHL antibody) at dilution of 1:100 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human liver cancer using 16538-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16538-1-AP (VHL antibody) at dilution of 1:100 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IF Staining of HepG2 using 16538-1-AP

  Immunofluorescent analysis of HepG2 cells, using VHL antibody 16538-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

16538-1-AP bindet in IHC, IF, ELISA VHL und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VHL fusion protein Ag9843
• Vollständiger Name: von Hippel-Lindau tumor suppressor
• Berechnetes Molekulargewicht: 172 aa, 20 kDa
• GenBank-Zugangsnummer: BC058831
• Gene symbol: VHL
• Gene ID (NCBI): 7428
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VHL (von Hippel-Lindau tumor suppressor) gene was identified as the tumor suppressor gene whose germ line mutations are associated with the inherited von Hippel-Lindau cancer syndrome (PMID: 8603073, 10722748). VHL patients develop a wide variety of tumors including retinal angioma, central nervous system hemangioblastoma, pheochromocytoma, and renal clear cell carcinoma (PMID: 10722748). VHL localizes predominantly to the cytoplasmic compartment but engages in a dynamic nuclear-cytoplasmic shuttle (PMID: 8700833, 9891082, 12101228). VHL has 3 isoforms with the molecular mass of 24, 20 and 18 kDa.

Publikationen

Species	Application	Title
human,mouse	IHC	Respir Res; Short isoform thymic stromal lymphopoietin reduces inflammation and aerobic glycolysis of asthmatic airway epithelium by antagonizing long isoform thymic stromal lymphopoietin.; Authors - Changhui Yu
human		Oncotarget; The oncoprotein HBXIP promotes glucose metabolism reprogramming via downregulating SCO2 and PDHA1 in breast cancer.; Authors - Fabao Liu
human	IHC	Front Oncol; Risk Scores Based on Six Survival-Related RNAs in a Competing Endogenous Network Composed of Differentially Expressed RNAs Between Clear Cell Renal Cell Carcinoma Patients Carrying Wild-Type or Mutant Von Hippel-Lindau Serve Well to Predict Malignancy and Prognosis.; Authors - Rui Zhu
human,mouse	IHC	Front Mol Biosci; Network-Based Expression Analyses and Experimental Verifications Reveal the Involvement of STUB1 in Acute Kidney Injury.; Authors - Yanting Shi
human,mouse	IHC,IF	Cancer Med; UBE2S promotes malignant properties via VHL/HIF-1α and VHL/JAK2/STAT3 signaling pathways and decreases sensitivity to sorafenib in hepatocellular carcinoma; Authors - Junyi Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jingwen (Verified Customer) (01-27-2020)

This antibody worked very well on the cancer samples by performing western blot and immunofluorescence.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 for WB, 1:100 for IF
• Cell Tissue Type: Cancer tissue

FH

Jie (Verified Customer) (01-27-2020)

achieved positive staining in liver cancer cells

• Applications: Western Blot,
• Primary Antibody Dilution: 1:200

FH

Lunfeng (Verified Customer) (01-27-2020)

GOOD

• Applications: Immunofluorescence,