VHL Polyklonaler Antikörper

VHL Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 24756-1-AP

Synonyme

HRCA1, Protein G7, pVHL, RCA1, VHL, VHL1

---

Galerie der Validierungsdaten

• 

  WB analysis using 24756-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24756-1-AP (VHL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 24756-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24756-1-AP (VHL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24756-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24756-1-AP (VHL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 24756-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24756-1-AP (VHL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24756-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24756-1-AP (VHL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24756-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24756-1-AP (VHL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 24756-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24756-1-AP (VHL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 24756-1-AP

  Immunofluorescent analysis of HepG2 cells using 24756-1-AP (VHL antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

24756-1-AP bindet in WB, IHC, IF, CoIP, ELISA VHL und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VHL fusion protein Ag21447
• Vollständiger Name: von Hippel-Lindau tumor suppressor
• Berechnetes Molekulargewicht: 172 aa, 20 kDa
• Beobachtetes Molekulargewicht: 18-24 kDa
• GenBank-Zugangsnummer: BC058831
• Gene symbol: VHL
• Gene ID (NCBI): 7428
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VHL (von Hippel-Lindau tumor suppressor) gene was identified as the tumor suppressor gene whose germ line mutations are associated with the inherited von Hippel-Lindau cancer syndrome (PMID: 8603073, 10722748). VHL patients develop a wide variety of tumors including retinal angioma, central nervous system hemangioblastoma, pheochromocytoma, and renal clear cell carcinoma (PMID: 10722748). VHL localizes predominantly to the cytoplasmic compartment but engages in a dynamic nuclear-cytoplasmic shuttle (PMID: 8700833, 9891082, 12101228). VHL has 3 isoforms with the molecular mass of 24, 20 and 18 kDa.

Publikationen

Species	Application	Title
human	WB	Front Oncol; Hypoxia-Induced LncRNA-MIR210HG Promotes Cancer Progression By Inhibiting HIF-1α Degradation in Ovarian Cancer.; Authors - Ping Liu
human	WB	Microvasc Res; A positive feedback loop between miR-574-3p and HIF-1α in promoting angiogenesis under hypoxia; Authors - Renwen Zhang
	CoIP	Cell Death Discov; Septin4 promotes cardiomyocytes apoptosis by enhancing the VHL-mediated degradation of HIF-1α.; Authors - Shaojun Wu
human	WB	EMBO J; A mitochondrial EglN1-AMPKα axis drives breast cancer progression by enhancing metabolic adaptation to hypoxic stress; Authors - Weiwei Jiang
human	IHC,WB	Cancer Res; Fatty Acid Oxidation Mediated by Malonyl-CoA Decarboxylase Represses Renal Cell Carcinoma Progression; Authors - Lijie Zhou
human	WB,CoIP	Pathol Oncol Res; MDM2 promotes cancer cell survival through regulating the expression of HIF-1α and pVHL in retinoblastoma; Authors - Shouhua Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

S (Verified Customer) (11-22-2021)

This antibody didn't work in my hands. There are too many non-specific bands to identify the VHL band.

• Applications: Western Blot

VHL Antibody Western Blot validation ( dilution) in (Cat no:24756-1-AP)